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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Chitobiase, Proteinase, Glycogen and some Trace Elements during Molting Cycle of Mud Crab (Scylla serrata Forsk?l 1775)) ผู้เขียน: ดร.จินตนา สและน้อย, รองศาสตราจารย์ ,  Jitlada Bootpugdeethum, ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ , นางอมรา ทองปาน, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractActivity profiles of chitobiase, proteinase and the content of glycogen and trace elements in gill, integument and haemolymph of mud crab (Scylla serrata) during molting cycle were determined. Chitobiase activity was at the highest level of 0.5467?0.0136 ?mol min-1mg protein-1 at 1-week premolt (D2) in gill and at 0.1413?0.0026 ?mol min-1 mg protein-1 in integument but reached the maximum level of 0.0851?0.0123 ?mol min-1mg protein-1 at 24-h postmolt (A2.2) in haemolymph. The high activities of proteinase, on the other hand, were found to be 0.6511 ? 0.0934 units mg protein-1 at 7-day postmolt (B2.3), 0.1553 ? 0.0419 units mg protein-1 at 1-week premolt (D2), and 0.1827?0.0110 units mg protein-1 at 12-h postmolt (A2.1) in gill, integument and haemolymph, respectively. These two enzymes are needed for carapace degradation as well as for chitin synthesis in preparation for carapace formation. For glycogen content, high accumulation of glycogen in the integument was observed throughout the molting cycle except at intermolt (C). In gill, however, the level of glycogen was high only before molting began (D2) while glycogen content in hepatopancreas and muscle, energy reserve tissues, was rather fluctuated but reached the maximum levels at 24-h postmolt (A2.2). As for trace elements, copper, a major component of hemocyanin, was found at high level at intermolt stage (C) in all tissues while magnesium, involving both nerve conduction and muscle contraction, showed the highest content at 7-day postmolt (B2.3) and 1-week premolt (D2) in integument and haemolymph, respectively. On the other hand, zinc showed high content at premolt stage in haemolymph and early postmolt stage in gill supporting its function in calcification. The results confirmed the roles of chitobiase, proteinase, glycogen, and trace elements which corresponded well with molting stages and physiological changes of mud crab. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Variations of Catalase and Glutathione Activities in Molting Cycle of Mud Crab (Scylla serrata)) ผู้เขียน:ดร.จินตนา สและน้อย, รองศาสตราจารย์, ภัทราวดี ศรีมีเทียน, ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe activities of the antioxidative enzyme, catalase (CAT), and the non-enzyme, glutathione (GSH) in 12 stages over the molting of mud crab (Scylla serrata) collected from Chanthaburi Province were determined. The stages were defined as: A1 (6-hour postmolt), A2.1 (12-hour postmolt), A2.2 (24-hour postmolt), B1 (2-day postmolt), B 2.1 (3-day postmolt), B2.2 ( 5-day postmolt), B2.3 (7-day postmolt), B2.4 (10 day postmolt), C (intermolt), D1 (2-week premolt), D2 (1- week premolt) and D3 (2-day premolt). The results revealed that CAT which controlled and eliminated excessive H2O2, increased in premolt (D1–D3) then dropped in the early postmolt stages (A1–A2.2) and increased again in the late postmolt stages (B1–B2.4). GSH, a reducing substance, which is also involved in the detoxification of H2O2 showed the same pattern in most tissues except in the hepatopancreas where it increased from the early postmolt stage (A1) and continued to increase through the postmolt stages. The variation in activity of CAT and GSH corresponded well with the metabolic activity through the molt cycle. |
 ที่มา:สำนักงานคณะกรรมการวิจัยแห่งชาติหัวเรื่อง:สารต้านอนุมูลอิสระและลิพิดเปอร์ออกซิเดสในวงจรการลอกคราบของปูทะเล (Scylla serrata Forskal 1775) หัวหน้าโครงการ:ดร.จินตนา สและน้อย, รองศาสตราจารย์ |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Variation of Calcium, N-acetylglucosamine, Glucosamine and Glucose Content during Molting Cycle of Mud Crab (Scylla serrata Forsk?l 1775)) ผู้เขียน:ดร.จินตนา สและน้อย, รองศาสตราจารย์, Jitlada Bootpugdeethum, ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์, นางอมรา ทองปาน, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractCalcium, N-acetylglucosamine, glucosamine and glucose content in the tissues of mud crab (Scylla serrata Forsk?l 1775) during molting cycle were determined. The results showed that calcium in gill decreased gradually from intermolt (C) to the lowest level of 6,527 ppm at 2-day premolt (D3) and increased again from 6-h postmolt (A1) to the highest level of 24,058.50 ppm at 7-day postmolt (B2.3) which was similar in pattern to calcium in the integument but of which at lower range (1,360.10-8,044.30 ppm). The highest content in haemolymph was found at 2-day premolt (D3). Variation of Nacetylglucosamine, a major component of chitin, showed similar pattern to calcium. Their content in gill and integument decreased from intermolt (C) to premolt (D1-D3) and increased at postmolt stages (A1-B2.3) while the pattern was opposite to that found in haemolymph which increased at premolt (D1- D3) and decreased at postmolt stages (A1-B2.3). For glucosamine, however, the content was quite low in both gill and integument but was moderately high at late postmolt in haemolymph. Glucose, on the other hand, seemed to be quite stable throughout the molting cycle (averaged 0.8973 ? 0.1489 % mg) in integument, but the profiles in hepatopancreas and gill were similar having the highest level at 5-day postmolt (B2.2). Glucose in muscle was at its peak of 0.7265 ? 0.1016 % mg at 2-day premolt (D3) followed by the ten times high level in haemolymph of 12.2020 ? 0.4807 % mg at late postmolt (B2.1). Variations of calcium, N-acetylglucosamine, glucosamine and glucose content corresponded to the changing of chemical composition of the tissues during chitin degradation of the old shed, chitin biosynthesis, mineralization of the new structure and the transportation of these substances between tissues. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Chitinase and Carbonic Anhydrase Activities during Molting Cycle
of Mud Crab (Scylla serrata Forskal 1775)) ผู้เขียน:ดร.จินตนา สและน้อย, รองศาสตราจารย์, ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์, ดร.อรุณี อิงคากุล, ผู้ช่วยศาสตราจารย์, ประทักษ์ ตาบทิพย์วรรณ, นางอมรา ทองปาน, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractOptimum conditions for the activities of chitinase and carbonic anhydrase in gill, integument and
haemolymph of mud crab (Scylla serrata Forskal 1775) were determined. The maximum activity of
chitinase in all 3 types of tissue was observed at pH 11 and 40?C. However, optimum conditions for
carbonic anhydrase in gill, integument and haemolymph were at pH 7.6 at 50?C, pH 7.2 at 50?C and pH
7.2 at 60?C, respectively. Both chitinase and carbonic anhydrase activities varied during various stages
of molting. For chitinase, activity in gill at 2-day premolt was significantly lower (P<0.01) than that at
6 hr to 5-day postmolt but not significantly (P<0.01) different from that at intermolt. Maximum chitinase
activity in integument observed at 24 hr postmolt was significantly higher (P<0.01) than that at intermolt
and early premolt. Chitinase activity in haemolymph was quite stable during premolt and postmolt but
significantly lower than that at intermolt (P<0.01). Observation of carbonic anhydrase activities in gill
and integument revealed significantly higher activity after molting than during intermolt and premolt
stages. Maximum activity of carbonic anhydrase in gill and integument was evident at 6 hr and 5-day
postmolt, respectively. For haemolymph, the level of carbonic anhydrase activities during various stages
of molting cycle were highly significantly different (P<0.01). The results indicated important roles of
chitinase and carbonic anhydrase at every stage in molting cycle of mud crab. |
ที่มา:มหาวิทยาลัยเกษตรศาสตร์หัวเรื่อง:องค์ประกอบทางชีวเคมีและกิจกรรมของเอนไซม์ในระบบภูมิคุ้มกันตลอดวงจรการลอกคราบของปูทะเล |
ที่มา:การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 46หัวเรื่อง:ปริมาณคาโรทีนอยด์ที่สะสมตลอดวงจรการลอกคราบของปูทะเล (Scylla serrata) |
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